Consilium MedicumConsilium Medicum2075-17532542-2170Consilium Medicum94627Research ArticlePossibilities of optimization of transfusion therapy in gastrosurgerySimutisI. Ssimutis@mail.ruBoyarinovG. A-MukhinA. Sprof.mukhin@mail.ruOtdelnovL. Aleonotdelnov@yandex.ruCity Clinical Hospital №40Nizhny Novgorod State Medical Academy of the Ministry of Health of the Russian Federation15082016188939528122021Copyright © 2016, Consilium Medicum2016Objective. To develop techniques pretransfusion rehabilitation canned red blood cells in patients with perioperative blood loss, severe. Materials and methods. The object of the study served as the red cells of various human blood storage time, stable preservative CPDA-1. Ozonation red blood cells was carried out by mixing it with ozonated solution NaCl 0.9% in an equivalent volume containing various concentrations of ozone, discrete increases of up to 20 mg/l. After 30 minutes of exposure to the slurry obtained erythrocyte suspension was determined concentration of malondialdehyde, adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), catalase activity in erythrocytes and their electrophoretic mobility. Results of the study. Under the influence of different ratios of ozone and erythrocytes detected ozone concentration range of 0.5-2 mg/l, causing an equivalent mixing restoration of ATP levels and 2,3-DPG in red blood cells to near normal values during the shelf life of 7 to 21 days. In terms of storage of packed red blood cells to 30 days, an increase 2,3-DPG content in red blood cells was observed on the background of the depletion of ATP pools in the cells. Thus inside the erythrocytes catalase activity in the basic group increases, resulting in 30 minutes after treatment, a significant reduction of malondialdehyde, without disturbing the electrophoretic mobility of treated erythrocytes. Conclusion. The work demonstrated the potential effects of ozone at low concentrations on key factors such as the system of oxygen transport and energy metabolic status of erythrocytes, as well as their morphological and functional properties (primarily deformability) at pretransfusion processing.2canned red cellsozoneadenosine triphosphate23-diphosphoglyceratemalondialdehydeконсервированная эритроцитарная массаозонаденозинтрифосфат3-дифосфоглицератмалоновый диальдегид[Гостищев В.К., Евсеев М.А. Гастродуоденальные кровотечения язвенной этиологии. Руководство для врачей. М.: ГЭОТАР-Медиа, 2008.][Малков И.С., Халикова Г.Р., Хамзин И.И. Об эффективности современных методов лечения больных с острыми кровотечениями из верхних отделов желудочно - кишечного тракта. Казанский мед. журн. 2010; 91 (3): 362-6.][Лобачева Г.В. Факторы риска развития ранних осложнений и их коррекция у больных после операции на открытом сердце. Дис.. д - ра мед. наук. М., 2000.][Мороз В.В., Кирсанова А.К., Новодержкина И.С. и др. Изменения ультраструктуры поверхности мембран эритроцитов после кровопотери и их коррекция лазерным облучением. Общая реаниматология. 2010; VI (2): 5-9.][d'Almeida M.S, Jagger J, Duggan M et al. A comparison of biochemical and functional alterations of rat and human erythrocytes stored in CPDA-1 for 29 days: implications for animal models of transfusion. Transfus Med 2000; 10: 291-303.][Tinmouth A, Fergusson D, Yee I.C, Hebert P.C. Clinical consequences of red cell storage in the critically ill. Transfusion 2006; 46: 2014-27.][Виноградова И.Л., Багрянцева С.Ю., Дервиз Г.В. Метод одновременного определения 2,3-ДФГ и АТФ в эритроцитах. Лаб. дело. 1980; 7: 424-6.]