Biotechnologies of plant resistance to viruses: CRISPR-Cas or rna interference

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Recently developed genetic technologies of gene editing (CRISPR-Cas) and RNA interference (RNAi) have already led to significant progress in almost all areas of life sciences, including biotechnology and medicine, and are now becoming increasingly popular in plant biology. In this review, we describe the basic principles of these two technologies and methods of their application on model plants and crops for the control of viral diseases. Examples of the antiviral effect of these technologies aimed at direct suppression of viral genomes of DNA and RNA-containing viruses, as well as suppression of the activity of host plant genes that increase susceptibility to viruses, are provided. The review contains a detailed comparison of the effectiveness of RNAi and CRISPR-Cas in protecting plants from viruses. The advantages and disadvantages of these technologies as well as the factors limiting their use in practice and ways to overcome them are discussed in detail.

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作者简介

N. Kalinina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

编辑信件的主要联系方式.
Email: kalinina@belozersky.msu.ru
俄罗斯联邦, 117997 Moscow; 119234 Moscow

N. Spechenkova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: kalinina@belozersky.msu.ru
俄罗斯联邦, 117997 Moscow

M. Taliansky

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: kalinina@belozersky.msu.ru
俄罗斯联邦, 117997 Moscow

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2. Fig. 1. Advantages and disadvantages of CRISPR-Cas and RNA interference technologies in plant protection against viral infections

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