Markers of conjugated octadecatrienoic acids in Raman spectra of vegetable oils: diagnostics of punicic and α-eleostearic acids

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Abstract

It is shown for the first time that using the method of Raman spectroscopy allows one to determine the content of conjugated octadecatrienoic (K-C18:3) acids in oil at their content of 8 wt. % at least. It is found that it is possible to reliably distinguish the isomers of the K-C18:3 acids containing conjugated (in punicic and α-eleostearic acids) and non-conjugated (in α-linolenic acid) C=C bonds by their Raman spectra. The obtained results can be used to develop efficient and non-destructive techniques for analyzing the composition and quality of oils, which contain conjugated octadecatrienic polyunsaturated fatty acids, and dietary supplements based on them.

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About the authors

S. M. Kuznetsov

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

V. S. Novikov

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

G. Yu. Nikolaeva

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

M. N. Moskovskiy

Federal Scientific Agroengineering Center VIM

Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

P. K. Laptinskaya

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

E. A. Sagitova

Prokhorov General Physics Institute of the Russian Academy of Sciences

Author for correspondence.
Email: sagitova@kapella.gpi.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Structural formulas (C18H30O2) of mutually isomeric punicic, α-eleostearic and α-linolenic acids. Molecular structures were calculated using density functional theory (DFT, see Materials and Methods). Carbon-carbon double bonds are shown in green.

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3. Fig. 2. Simulated Raman spectra of punicic, α-eleostearic and α-linolenic acids and the experimental Raman spectrum of the Omega-5 dietary supplement.

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4. Fig. 3. Raman spectra of vegetable oils in the range of 600–1800 cm–1: blue and red curves are the spectra of samples containing punicic and α-eleostearic (K-C18:3) PUFA, respectively; black curves are oils that do not contain K-C18:3 PUFA.

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5. Fig. 4. Raman spectra of the studied vegetable oils and Omega-5 dietary supplements in the regions of (a) 2650–3200 cm–1 and (b) 2985–3075 cm–1. Blue and red curves are the spectra of samples containing punicic and α-eleostearic acids, respectively; black curves are oils that lack K-C18:3 PUFA.

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